|
Status |
Public on Mar 01, 2009 |
Title |
N2 SDC-2 HD-IP1 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
N2 embryo input
|
Organism |
Caenorhabditis elegans |
Characteristics |
N2 XX embryo nonselected input
|
Treatment protocol |
Embryos used for microarray analysis were obtained from bleached gravid adults.
|
Growth protocol |
Wild type, N2, Caenorhabditis elegans were grown on NG pates suplimented with 100 µg/ml carbinicillin and 1 µg/ml IPTG seeded with E. coli HT115 (DE3)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
see PDF linked to GSE14640
|
Label |
Cy3
|
Label protocol |
Sample labeling protocol provided and performed by the FHCRC Genomics Facility using standard NimbleGen protocol
|
|
|
Channel 2 |
Source name |
N2 embryo SDC-2 IP
|
Organism |
Caenorhabditis elegans |
Characteristics |
N2 XX embryo SDC-2 IP
|
Treatment protocol |
Embryos used for microarray analysis were obtained from bleached gravid adults.
|
Growth protocol |
Wild type, N2, Caenorhabditis elegans were grown on NG pates suplimented with 100 µg/ml carbinicillin and 1 µg/ml IPTG seeded with E. coli HT115 (DE3)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
see PDF linked to GSE14640
|
Label |
Cy5
|
Label protocol |
Sample labeling protocol provided and performed by the FHCRC Genomics Facility using standard NimbleGen protocol
|
|
|
|
Hybridization protocol |
Hybridization protocol provided and performed by the FHCRC Genomics Facility using standard NimbleGen protocol
|
Scan protocol |
Sample scan protocol provided and performed by the FHCRC Genomics Facility using standard NimbleGen protocol
|
Description |
N/A
|
Data processing |
performed at Nimblegen using their standard protocol producing gff files with log2 ratios of experimental/input (http://www.nimblegen.com/products/chip/index.html).
|
|
|
Submission date |
Jan 30, 2009 |
Last update date |
Feb 02, 2009 |
Contact name |
Barbara J. Meyer |
E-mail(s) |
bjmeyer@berkeley.edu
|
Phone |
510 643 5583
|
Organization name |
HHMI/UCB
|
Department |
MCB
|
Lab |
Meyer
|
Street address |
16 Barker Hall #3204
|
City |
Berkeley |
State/province |
CA |
ZIP/Postal code |
94720 |
Country |
USA |
|
|
Platform ID |
GPL8134 |
Series (2) |
GSE14640 |
A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome |
GSE14652 |
DCC binding and function (ChIP-chip: SDC-2) |
|