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Sample GSM362604 Query DataSets for GSM362604
Status Public on Apr 01, 2013
Title MuRF1 +/+ // MuRF2 +/+: Biological replicate 1.
Sample type RNA
 
Channel 1
Source name Total RNA from murine heart labeled with Cyanine-5 (red).
Organism Mus musculus
Characteristics Cardiac tissue
untreated
Treatment protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen All-Prep kit following manufacturer's instructions
Label Cy5
Label protocol 50 ng of total RNA were amplified and labeled with Cy3-CTP using the Agilent Low Input RNA amplification kit exactly as described by the manufacturer.
 
Channel 2
Source name Total RNA from pooled whole mouse neonates (day 1), labeled with Cyanine-3 (green).
Organism Mus musculus
Characteristics Strain: C57BL/6
Age: postnatal Day 1
Tissue: whole pup
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen All-Prep kit following manufacturer's instructions
Label Cy3
Label protocol 50 ng of total RNA were amplified and labeled with Cy3-CTP using the Agilent Low Input RNA amplification kit exactly as described by the manufacturer.
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
Scan protocol Scanned on an Axon Instruments GenePix 4000b scanner.
Images were quantified using Genepix Pro (version 6.0).
Description MuRF1 +/+ // MuRF2 +/+: Biological replicate 1.
Data processing LOWESS normalized, background subtracted data obtained from processed Red signal/processed Green signal by Genepix Pro V 6.0. Data was subsequently downloaded and filtered for features present in less than 70% of arrays.
 
Submission date Jan 22, 2009
Last update date Apr 01, 2013
Contact name Monte Shaw Willis
E-mail(s) monte_willis@med.unc.edu
Phone 919-843-1938
Organization name University of North Carolina
Department Pathology & Laboratory Medicine
Street address 103 Mason Farm Road, MBRB 2340B
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7525
Country USA
 
Platform ID GPL7202
Series (1)
GSE14512 Muscle Ring Finger 1 (MuRF1) and MuRF2 are Necessary but Functionally Redundant During Developmental Cardiac Growth and Regulate E2F1-Mediated Gene Expression In Vivo

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5)

Data table
ID_REF VALUE
A_52_P756921 0.023936316
A_52_P1133703 -0.14577723
A_52_P1052870 -0.17459562
A_52_P1021909 -0.072758436
A_51_P133060 0.004793957
A_51_P287221 1.0100245
A_52_P112159 0.08956711
A_51_P378063 1.024194
A_52_P289204 -0.123961866
A_51_P439156 0.6628742
A_52_P2181 0.6072991
A_52_P53154 -0.012082864
A_52_P527925 0.058469772
A_52_P315910 -0.014326006
A_52_P519870 0.6111902
A_52_P96087 1.1265098
A_51_P137808 0.31706333
A_52_P552026 -0.022881418
A_52_P42395 0.038750112
A_52_P350876 -0.33342987

Total number of rows: 41174

Table truncated, full table size 982 Kbytes.




Supplementary file Size Download File type/resource
GSM362604.txt.gz 12.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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