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Sample GSM362603 Query DataSets for GSM362603
Status Public on Apr 01, 2013
Title MuRF1 -/- // MuRF2 -/-: Biological replicate 2.
Sample type RNA
 
Channel 1
Source name Total RNA from murine heart labeled with Cyanine-5 (red).
Organism Mus musculus
Characteristics Cardiac tissue
untreated
Treatment protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen All-Prep kit following manufacturer's instructions
Label Cy5
Label protocol 50 ng of total RNA were amplified and labeled with Cy3-CTP using the Agilent Low Input RNA amplification kit exactly as described by the manufacturer.
 
Channel 2
Source name Total RNA from pooled whole mouse neonates (day 1), labeled with Cyanine-3 (green).
Organism Mus musculus
Characteristics Strain: C57BL/6
Age: postnatal Day 1
Tissue: whole pup
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen All-Prep kit following manufacturer's instructions
Label Cy3
Label protocol 50 ng of total RNA were amplified and labeled with Cy3-CTP using the Agilent Low Input RNA amplification kit exactly as described by the manufacturer.
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
Scan protocol Scanned on an Axon Instruments GenePix 4000b scanner.
Images were quantified using Genepix Pro (version 6.0).
Description MuRF1 -/- // MuRF2 -/-: Biological replicate 2.
Data processing LOWESS normalized, background subtracted data obtained from processed Red signal/processed Green signal by Genepix Pro V 6.0. Data was subsequently downloaded and filtered for features present in less than 70% of arrays.
 
Submission date Jan 22, 2009
Last update date Apr 01, 2013
Contact name Monte Shaw Willis
E-mail(s) monte_willis@med.unc.edu
Phone 919-843-1938
Organization name University of North Carolina
Department Pathology & Laboratory Medicine
Street address 103 Mason Farm Road, MBRB 2340B
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7525
Country USA
 
Platform ID GPL7202
Series (1)
GSE14512 Muscle Ring Finger 1 (MuRF1) and MuRF2 are Necessary but Functionally Redundant During Developmental Cardiac Growth and Regulate E2F1-Mediated Gene Expression In Vivo

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5)

Data table
ID_REF VALUE
A_52_P756921 0.029270798
A_52_P1133703 -0.26582235
A_52_P1052870 -0.020748854
A_52_P1021909 0.14104533
A_51_P133060 -0.01204231
A_51_P287221 0.09660427
A_52_P112159 -0.1358166
A_51_P378063 -2.0972826
A_52_P289204 0.40612248
A_51_P439156 -0.5871343
A_52_P2181 -0.828531
A_52_P53154 -0.04265332
A_52_P527925 -0.8979831
A_52_P315910 -0.104849264
A_52_P519870 -1.8742275
A_52_P96087 -1.3614566
A_51_P137808 0.3923087
A_52_P552026 0.583715
A_52_P42395 2.8132188
A_52_P350876 -0.26868007

Total number of rows: 41174

Table truncated, full table size 970 Kbytes.




Supplementary file Size Download File type/resource
GSM362603.txt.gz 10.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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