|Public on Apr 11, 2019
|Control 223 (C7)
|ex vivo corneal endothelium
|cell type: endothelial
|Cultured cells were grown or Human Endothelial SFM.
|TriReagent extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNeasy Clean-Up Kit.
Enrichment for poly(A) RNAs was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module, followed by library preparation using the PrepX Complete ILM DNA Library Kit. Libraries were prepared as per the manufacturer's instructions.
|Illumina HiSeq 3000
|The FASTQ files containing the RNA-sequencing data were aligned with CLC Biomedical Genomic Workbench to the hg38 genome.
Using CLC Biomedical Genomic Workbench, reads and read-depth were transformed to reads per kilobase per million (RPKM) values, a normalized quantity that accounts for gene size.
These data were annotated using the Ensemble 88 transcript database.
Supplementary_files_format_and_content: The processed data file is a txt file with Ensemble 88 gene symbol ID in the first column. Sample names are given on the first row with RPKM values for each gene running down the column.
|Feb 13, 2019
|Last update date
|Apr 11, 2019
|Anthony John Aldave
|University of California, Los Angeles
|200 Stein Plaza, DS 3-143
|Alterations in GRHL2-OVOL2-ZEB1 Axis and Aberrant Activation of Wnt Signaling Lead to Altered Gene Transcription in Posterior Polymorphous Corneal Dystrophy