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Sample GSM3601944 Query DataSets for GSM3601944
Status Public on Feb 13, 2019
Title MV4;11 70 mg/kg CBL0137 treated replicate 1
Sample type SRA
Source name MV4;11 cells were inoculated subcutaneously in the flank of NOD/SCID (NOD/SCID NOD.CB17-Prkdcscid/SzJ) mice.
Organisms Homo sapiens; Mus musculus
Characteristics tissue: Subcutaneous MV4;11 tumor
treatment: treated with curaxin CBL0137
Treatment protocol > 1 million MV4;11 cells were inoculated subcutaneously in the flank of NOD/SCID (NOD/SCID NOD.CB17-Prkdcscid/SzJ) mice. When tumors measured around 100 mm3, mice were treated with CBL0137 (70 mg/kg CBL0137 intravenously) on day 0 and day 8. Tumors were harvested 24h post last injection on day 8. Tumors were also harvested from untreated mice. Tumors were flash frozen in liquid nitrogen and RNA was isolated.
Extracted molecule total RNA
Extraction protocol RNA was isolated with Trizol
RNA-seq libraries were generated using Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold prep kit using 1 μg of RNA input
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description RNA sequencing data of MV4;11 CBL0137 treated replicate 1
Data processing Illumina paired-end RNA sequencing data was aligned using STAR to the human genome assembly hg19 and mouse genome assembly mm10
BAM files were sorted and index using SAMtools
Gene expression and isoform expression counts were calculated using RSEM
Genome_build: hg19 and mm10
Supplementary_files_format_and_content: txt file contains the TPM counts for each gene in each of the samples
Submission date Feb 12, 2019
Last update date Feb 13, 2019
Contact name Michelle Haber
Organization name Children's Cancer Institute
Street address Lowy Cancer Research Centre, PO BOX 81
City Randwick
State/province NSW
ZIP/Postal code 2031
Country Australia
Platform ID GPL22245
Series (1)
GSE126442 Gene expression analysis of a subcutaneous MV4;11 tumor treated with curaxin CBL0137.
BioSample SAMN10920287
SRA SRX5367839

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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