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Sample GSM359321 Query DataSets for GSM359321
Status Public on Dec 01, 2012
Title Serum_T1DH_7059
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics T1D
IL-1Ra High
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNeasy minikit
Label Cy3
Label protocol Total RNA (200 ng) was coverted into biotin labeled cRNA using Target Amp cDNA synthesis kit (Epicenter catalog no. TAB1R6924).
 
Hybridization protocol Approximately 750ng of labeled cRNA was fragmented, and hybridized to the Illumina HumanRef-8 v3.0 beadchips in accordance to the Illumina technical manual. RNA from each culture was independently analyzed.
Scan protocol After hybridization, arrays were washed, stained with Cy3-conjugated streptavidin, and scanned. Image data were analyzed with BeadStudio V2.0.
Description STH7059
Data processing The raw data were normalized using Robust Spline Normalization.
 
Submission date Jan 09, 2009
Last update date Dec 01, 2012
Contact name Jin-Xiong She
E-mail(s) jshe@gru.edu
Phone 706-721-3410
Fax 706-721-3688
URL http://www.cbgm.mcg.edu/
Organization name Medical College of Georgia
Department Center for Biotechnology and Genomic Medicine
Street address 1120 15th Street, CA4124
City Augusta
State/province GA
ZIP/Postal code 30912-2400
Country USA
 
Platform ID GPL6883
Series (1)
GSE14368 The anti-inflammatory IL-1 receptor antagonist (IL-1ra) protects against the development of islet autoimmunity.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
ILMN_1802380 10.71179687
ILMN_1792389 7.583257045
ILMN_2375156 8.036191413
ILMN_1697642 9.395883362
ILMN_1681845 11.35653169
ILMN_1690979 7.498247238
ILMN_1811114 7.35920734
ILMN_1660729 7.295627437
ILMN_2129572 12.61420854
ILMN_1705659 7.379455541
ILMN_1797055 7.733202568
ILMN_1670547 7.424008733
ILMN_2342515 8.743623371
ILMN_1800425 10.9610468
ILMN_1783852 11.50507266
ILMN_1721344 11.14106342
ILMN_1679973 7.509528249
ILMN_1701854 11.81006774
ILMN_1678707 11.82168882
ILMN_2276504 7.364380852

Total number of rows: 24526

Table truncated, full table size 596 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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