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Sample GSM359315 Query DataSets for GSM359315
Status Public on Dec 01, 2012
Title Serum_AbNH_8058
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics Antibody Negative
IL-1Ra High
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNeasy minikit
Label Cy3
Label protocol Total RNA (200 ng) was coverted into biotin labeled cRNA using Target Amp cDNA synthesis kit (Epicenter catalog no. TAB1R6924).
 
Hybridization protocol Approximately 750ng of labeled cRNA was fragmented, and hybridized to the Illumina HumanRef-8 v3.0 beadchips in accordance to the Illumina technical manual. RNA from each culture was independently analyzed.
Scan protocol After hybridization, arrays were washed, stained with Cy3-conjugated streptavidin, and scanned. Image data were analyzed with BeadStudio V2.0.
Description SAH8058
Data processing The raw data were normalized using Robust Spline Normalization.
 
Submission date Jan 09, 2009
Last update date Dec 01, 2012
Contact name Jin-Xiong She
E-mail(s) jshe@gru.edu
Phone 706-721-3410
Fax 706-721-3688
URL http://www.cbgm.mcg.edu/
Organization name Medical College of Georgia
Department Center for Biotechnology and Genomic Medicine
Street address 1120 15th Street, CA4124
City Augusta
State/province GA
ZIP/Postal code 30912-2400
Country USA
 
Platform ID GPL6883
Series (1)
GSE14368 The anti-inflammatory IL-1 receptor antagonist (IL-1ra) protects against the development of islet autoimmunity.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
ILMN_1802380 10.77515502
ILMN_1792389 7.591522571
ILMN_2375156 7.733880829
ILMN_1697642 9.267392109
ILMN_1681845 11.72884952
ILMN_1690979 7.553333369
ILMN_1811114 7.410711942
ILMN_1660729 7.371014271
ILMN_2129572 14.38117574
ILMN_1705659 7.429624144
ILMN_1797055 7.94398632
ILMN_1670547 7.362161225
ILMN_2342515 8.788406845
ILMN_1800425 10.66994276
ILMN_1783852 12.03086321
ILMN_1721344 10.92780502
ILMN_1679973 7.443609904
ILMN_1701854 11.57059394
ILMN_1678707 12.09510972
ILMN_2276504 7.374761604

Total number of rows: 24526

Table truncated, full table size 595 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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