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Sample GSM358214 Query DataSets for GSM358214
Status Public on Jan 08, 2009
Title Normal, biological rep 6
Sample type RNA
 
Source name Normal liver tissue
Organism Homo sapiens
Characteristics Tissue: Normal
Treatment protocol Five mm frozen section slides were made and stained with H&E for Pathologist evaluation to determine tumor content. After Pathologist evaluation, normal/necrotic tissue was macrodissected. Twenty 20 mm sections were performed in a 15 ml tube with 10 ml of Trizol.
Growth protocol Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Liver tissue samples were collected in liquid nitrogen or RNA later solution (Ambion, Inc, Austin, TX), and stored at -80°C until use.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.The precipitated product was further purified using RNeasy Mini Kit (Qiagen, CA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip® Scanner 3000 by Affymetrix.
Description Gene expression using RMA method
Data processing The CEL files from the two Affymetrix series were combined using the matchprobes package in the R programming environment. Thereafter, the RMA method in the R affy package was used to obtain probe set expression summaries.
 
Submission date Jan 07, 2009
Last update date Jan 07, 2009
Contact name Kellie J. Archer
E-mail(s) archer.43@osu.edu
Phone (614) 247-6167
Organization name Ohio State University
Department Division of Biostatistics
Street address 1841 Neil Ave
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
 
Platform ID GPL571
Series (1)
GSE14323 RMA expression data for liver samples from subjects with HCV, HCV-HCC, or normal liver

Data table header descriptions
ID_REF
VALUE Robust multiarray average (RMA)

Data table
ID_REF VALUE
1007_s_at 6.218602133
1053_at 6.403828827
117_at 5.962045685
121_at 8.347587435
1255_g_at 4.206341942
1294_at 7.129837836
1316_at 5.844304533
1320_at 5.191810014
1405_i_at 4.589698645
1431_at 13.29538245
1438_at 5.91629687
1487_at 8.162918372
1494_f_at 11.48057862
1598_g_at 8.072844871
160020_at 7.427369837
1729_at 7.211179337
1773_at 5.644196268
177_at 5.273331545
179_at 9.227646226
1861_at 5.349874987

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM358214.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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