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Sample GSM3573456 Query DataSets for GSM3573456
Status Public on Jan 22, 2019
Title HepG2_control (2nd)
Sample type RNA
 
Source name HepG2, negative control miRNA mimic, 72 hours
Organism Homo sapiens
Characteristics cell line: HepG2
cell type: hepatocellular carcinoma
transfection: negative control miRNA
Treatment protocol For characterization of miR-493-5p targets, total RNAs were collected from Hep3B and HepG2 cells 72 hours after cell transfection using miR-493-5p and negative control miRNA mimics (100 nM).
Growth protocol Human Hep3B and HepG2 cells were purchased from the American Type Culture Collection and maintained in DMEM (Gibco) supplemented with penicillin (50 IU/mL; Gibco), streptomycin (50 µg/mL; Gibco), and 10 % fetal bovine serum (FBS; Thermo Scientific).
Extracted molecule total RNA
Extraction protocol mRNA and miRNA were purified using a miRNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. Total RNA was quantified using a NanoDrop 1000 spectrophotometer (Thermo Scientific), and the integrity of the RNA was evaluated with an Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol. Cyanine-3 (Cy3)
 
Hybridization protocol Agilent one-color gene expression hyb/wash protocol.
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
Description Gene expression after miR-493-5p overexpression versus control
Data processing The raw data were processed using Agilent’s Feature Extraction Software version 10.7.3.1 to analyze the array and calculate the intensities of the measured spots.
 
Submission date Jan 21, 2019
Last update date Jan 22, 2019
Contact name Luc Gailhouste
E-mail(s) luc.gailhouste@riken.jp
Organization name RIKEN
Department Cluster for Pioneering Research
Lab Liver Cancer Prevention Research Unit
Street address 408 Main Research Building, 2-1 Hirosawa
City Wako
State/province Saitama
ZIP/Postal code 351-0198
Country Japan
 
Platform ID GPL17077
Series (1)
GSE125407 Characterization of microRNA-493-5p targets in liver cell lines (2nd set of data)

Data table header descriptions
ID_REF
VALUE Normalized signal intensity.

Data table
ID_REF VALUE
A_23_P117082 -0.003610611
A_33_P3246448 -0.3605733
A_33_P3318220 -0.35866165
A_33_P3236322 -0.47531796
A_33_P3319925 0.28401947
A_21_P0000509 0.6272106
A_21_P0000744 -0.564024
A_24_P215804 0.6301503
A_23_P110167 1.0353246
A_33_P3211513 -0.17363977
A_23_P103349 -0.17614412
A_32_P61480 -0.25942516
A_33_P3788124 -0.25877857
A_33_P3414202 -0.003550053
A_33_P3316686 -0.26651144
A_33_P3300975 -0.40416622
A_33_P3263061 -0.49919796
A_33_P3261373 -0.40188885
A_24_P278460 -0.3019309
A_21_P0013109 -0.39989567

Total number of rows: 50737

Table truncated, full table size 1224 Kbytes.




Supplementary file Size Download File type/resource
GSM3573456_3-G2_Ctrl.txt.gz 12.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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