|
| Status |
Public on Dec 25, 2008 |
| Title |
S2 dicer knockdown,biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
S2 cells treated with dicer dsRNA
|
| Organism |
Drosophila melanogaster |
| Characteristics |
S2, dicer dsRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
cDNA was hybridized according to manufacturer's protocol on GeneChip Drosophila Tiling 1.0R. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Tiling arrays were scanned using the GeneChip Scanner 3000
|
| Description |
S2 dicer RNAi
|
| Data processing |
Data was processed using the Model-based Analysis of Tiling-array (MAT) software package (Johnson et al., 2006). RNA arrays were analyzed using a bandwidth size of 150, minimum probe gap of 300, probe number of 5 and p-value threshold of 1e-05.
|
| |
|
| Submission date |
Dec 24, 2008 |
| Last update date |
Dec 24, 2008 |
| Contact name |
Joseph Rodriguez |
| Organization name |
Brandeis University
|
| Department |
Biology
|
| Lab |
Rosbash
|
| Street address |
415 South Street
|
| City |
Waltham |
| ZIP/Postal code |
02453 |
| Country |
USA |
| |
|
| Platform ID |
GPL5919 |
| Series (1) |
| GSE14215 |
Genome wide identification of targets of the drosha-pasha/DGCR8 complex |
|
