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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 25, 2008 |
| Title |
S2 control,biological replicate 1 |
| Sample type |
RNA |
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| Source name |
S2 cells, control
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| Organism |
Drosophila melanogaster |
| Characteristics |
S2, control
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| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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| Label |
biotin
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| Label protocol |
cDNA was prepared according to the standard Affymetrix protocol
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| Hybridization protocol |
cDNA was hybridized according to manufacturer's protocol on GeneChip Drosophila Tiling 1.0R. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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| Scan protocol |
Tiling arrays were scanned using the GeneChip Scanner 3000
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| Description |
S2, control
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| Data processing |
Data was processed using the Model-based Analysis of Tiling-array (MAT) software package (Johnson et al., 2006). RNA arrays were analyzed using a bandwidth size of 150, minimum probe gap of 300, probe number of 5 and p-value threshold of 1e-05.
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| Submission date |
Dec 24, 2008 |
| Last update date |
Dec 24, 2008 |
| Contact name |
Joseph Rodriguez |
| Organization name |
Brandeis University
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| Department |
Biology
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| Lab |
Rosbash
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| Street address |
415 South Street
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| City |
Waltham |
| ZIP/Postal code |
02453 |
| Country |
USA |
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| Platform ID |
GPL5919 |
| Series (1) |
| GSE14215 |
Genome wide identification of targets of the drosha-pasha/DGCR8 complex |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM356322.CEL.gz |
22.1 Mb |
(ftp)(http) |
CEL |
| GSM356322.bar.gz |
18.2 Mb |
(ftp)(http) |
BAR |
| Processed data provided as supplementary file |
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