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Sample GSM355341 Query DataSets for GSM355341
Status Public on Jun 01, 2011
Title Training77-2
Sample type RNA
 
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
This sample uses the same CEL data as GSM355251
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
 
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
E-mail(s) JEGreen@mail.nih.gov
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL571
Series (2)
GSE14209 Dysregulated genes associated with acquired resistance
GSE14210 Expression data from human endoscopic biopsy samples
Relations
Alternative to GSM355251

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 10.68711305
1053_at 8.491082753
117_at 6.602475575
121_at 8.380566363
1255_g_at 4.452435343
1294_at 7.917059304
1316_at 6.445198071
1320_at 5.295592172
1405_i_at 7.506815775
1431_at 4.444600036
1438_at 7.70004606
1487_at 8.865933056
1494_f_at 6.130976641
1598_g_at 9.891242456
160020_at 8.392058882
1729_at 8.207038861
177_at 5.290057441
1773_at 5.989552696
179_at 8.858265956
1861_at 6.589837657

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM355341.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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