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Sample GSM355335 Query DataSets for GSM355335
Status Public on Jun 01, 2011
Title Training52-2
Sample type RNA
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
This sample uses the same CEL data as GSM355226
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL571
Series (2)
GSE14209 Dysregulated genes associated with acquired resistance
GSE14210 Expression data from human endoscopic biopsy samples
Alternative to GSM355226

Data table header descriptions

Data table
1007_s_at 10.39323104
1053_at 8.155623209
117_at 7.919083801
121_at 8.007468404
1255_g_at 4.823558745
1294_at 7.787161501
1316_at 6.039270709
1320_at 5.414086378
1405_i_at 7.283227228
1431_at 5.632570154
1438_at 8.334594905
1487_at 8.377187063
1494_f_at 5.968077092
1598_g_at 9.304535955
160020_at 8.674959083
1729_at 8.536313121
177_at 5.921204357
1773_at 5.787644675
179_at 8.191259708
1861_at 6.325843835

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM355335.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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