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Sample GSM355334 Query DataSets for GSM355334
Status Public on Jun 01, 2011
Title Training50-2
Sample type RNA
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
This sample uses the same CEL data as GSM355224
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL571
Series (2)
GSE14209 Dysregulated genes associated with acquired resistance
GSE14210 Expression data from human endoscopic biopsy samples
Alternative to GSM355224

Data table header descriptions

Data table
1007_s_at 10.04102094
1053_at 8.991258235
117_at 7.094253355
121_at 8.466991383
1255_g_at 4.162058237
1294_at 7.619297276
1316_at 6.013436559
1320_at 5.74255222
1405_i_at 9.035637114
1431_at 4.94052922
1438_at 6.498216712
1487_at 8.81838117
1494_f_at 6.063051292
1598_g_at 9.228976338
160020_at 8.601717637
1729_at 8.90024033
177_at 5.540818337
1773_at 5.556610656
179_at 8.683231269
1861_at 7.239965397

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM355334.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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