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Sample GSM355332 Query DataSets for GSM355332
Status Public on Jun 01, 2011
Title Training46-2
Sample type RNA
 
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
This sample uses the same CEL data as GSM355220
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
 
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
E-mail(s) JEGreen@mail.nih.gov
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL571
Series (2)
GSE14209 Dysregulated genes associated with acquired resistance
GSE14210 Expression data from human endoscopic biopsy samples
Relations
Alternative to GSM355220

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 10.86209925
1053_at 8.067354687
117_at 6.66491484
121_at 7.777857073
1255_g_at 4.365100046
1294_at 8.377670128
1316_at 6.081385439
1320_at 5.774395761
1405_i_at 8.428227782
1431_at 4.378429846
1438_at 7.529194804
1487_at 8.8739142
1494_f_at 5.816227486
1598_g_at 9.6214682
160020_at 8.546278338
1729_at 8.546978281
177_at 6.420500611
1773_at 6.076560008
179_at 8.703334601
1861_at 7.853931073

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM355332.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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