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Sample GSM355331 Query DataSets for GSM355331
Status Public on Jun 01, 2011
Title Training43-2
Sample type RNA
 
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
This sample uses the same CEL data as GSM355217
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
 
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
E-mail(s) JEGreen@mail.nih.gov
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL571
Series (2)
GSE14209 Dysregulated genes associated with acquired resistance
GSE14210 Expression data from human endoscopic biopsy samples
Relations
Alternative to GSM355217

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 11.60916813
1053_at 7.712889884
117_at 6.620042806
121_at 8.932895198
1255_g_at 4.818818857
1294_at 7.782026731
1316_at 6.552786811
1320_at 5.474393111
1405_i_at 7.595794055
1431_at 4.676984406
1438_at 6.147957433
1487_at 9.28489094
1494_f_at 6.554414736
1598_g_at 9.19948912
160020_at 8.682100574
1729_at 8.922793256
177_at 6.776480575
1773_at 5.595083689
179_at 9.204075793
1861_at 5.94566982

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM355331.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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