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Sample GSM355259 Query DataSets for GSM355259
Status Public on Jun 01, 2011
Title Training85
Sample type RNA
 
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
 
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
E-mail(s) JEGreen@mail.nih.gov
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL571
Series (2)
GSE14208 Clinical response of metastatic gastric cancer patients to cisplatin and fluorouracil (CF) combination chemotherapy
GSE14210 Expression data from human endoscopic biopsy samples

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 11.03040343
1053_at 8.77363364
117_at 5.732699624
121_at 7.767767548
1255_g_at 3.776033449
1294_at 6.315283489
1316_at 5.100516552
1320_at 4.79525597
1405_i_at 5.990795429
1431_at 4.213186113
1438_at 5.68268025
1487_at 8.155737465
1494_f_at 5.541089466
1598_g_at 8.073732947
160020_at 7.008042514
1729_at 7.802545589
177_at 5.333227637
1773_at 4.905414305
179_at 7.462578763
1861_at 6.301896572

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM355259.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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