|
Status |
Public on Jun 19, 2019 |
Title |
14_Day22_2 |
Sample type |
SRA |
|
|
Source name |
Kidney organoid derived from MAFB-P2A-eGFP H9 hESC, collected on differentiation day 22, batch 2
|
Organism |
Homo sapiens |
Characteristics |
cell line source: H9 source cell type: human embryonic stem cells differentiation day: Day 22 cell line of origin: H9 human embryonic stem cell genotype/variation: MAFB-P2A-eGFP
|
Treatment protocol |
NA
|
Growth protocol |
Kidney differentiation followed protocol described in Morizane et al., 2016
|
Extracted molecule |
total RNA |
Extraction protocol |
Ambion Recoverall Total Nucleic Acid Isolation Kit and QIAGEN Rneasy Micro Kit; Qiagen Rneasy micro kit Kapa stranded mRNA-Seq library (#KK8420)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
processed data file: TPM-table_progression_org_differentiation_all-reps.txt
|
Data processing |
Fastq files were aligned to hg38 with TopHat2 Gene-level quantification was performed with Partek E/M through Partek Genomic Suite Gene-level read couns was normalized to TPM through Partek Genomic Suite Genome_build: hg38 Supplementary_files_format_and_content: TPM
|
|
|
Submission date |
Dec 26, 2018 |
Last update date |
Jun 19, 2019 |
Contact name |
Andrew P McMahon |
E-mail(s) |
amcmahon@med.usc.edu
|
Phone |
323-442-7847
|
Organization name |
University of Southern California
|
Department |
Stem Cell Biol & Regen Med
|
Lab |
Andrew McMahon
|
Street address |
1425 San Pablo St, BCC 312
|
City |
Los Angeles |
State/province |
California |
ZIP/Postal code |
90033 |
Country |
USA |
|
|
Platform ID |
GPL18573 |
Series (1) |
GSE124392 |
In vivo developmental trajectories of human podocyte development inform in vitro differentiation of pluripotent stem-cell derived podocytes |
|
Relations |
BioSample |
SAMN10645029 |
SRA |
SRX5180676 |