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Sample GSM3531619 Query DataSets for GSM3531619
Status Public on Jun 19, 2019
Title 14_Day22_2
Sample type SRA
 
Source name Kidney organoid derived from MAFB-P2A-eGFP H9 hESC, collected on differentiation day 22, batch 2
Organism Homo sapiens
Characteristics cell line source: H9
source cell type: human embryonic stem cells
differentiation day: Day 22
cell line of origin: H9 human embryonic stem cell
genotype/variation: MAFB-P2A-eGFP
Treatment protocol NA
Growth protocol Kidney differentiation followed protocol described in Morizane et al., 2016
Extracted molecule total RNA
Extraction protocol Ambion Recoverall Total Nucleic Acid Isolation Kit and QIAGEN Rneasy Micro Kit; Qiagen Rneasy micro kit
Kapa stranded mRNA-Seq library (#KK8420)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description processed data file:
TPM-table_progression_org_differentiation_all-reps.txt
Data processing Fastq files were aligned to hg38 with TopHat2
Gene-level quantification was performed with Partek E/M through Partek Genomic Suite
Gene-level read couns was normalized to TPM through Partek Genomic Suite
Genome_build: hg38
Supplementary_files_format_and_content: TPM
 
Submission date Dec 26, 2018
Last update date Jun 19, 2019
Contact name Andrew P McMahon
E-mail(s) amcmahon@med.usc.edu
Phone 323-442-7847
Organization name University of Southern California
Department Stem Cell Biol & Regen Med
Lab Andrew McMahon
Street address 1425 San Pablo St, BCC 312
City Los Angeles
State/province California
ZIP/Postal code 90033
Country USA
 
Platform ID GPL18573
Series (1)
GSE124392 In vivo developmental trajectories of human podocyte development inform in vitro differentiation of pluripotent stem-cell derived podocytes
Relations
BioSample SAMN10645029
SRA SRX5180676

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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