|
| Status |
Public on Dec 27, 2018 |
| Title |
WT2: Control Embyronic Stem Cell Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Embyronic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Embyronic Stem Cell
genotype: wild type
|
| Growth protocol |
ES cells were kept in an undifferentiated, pluripotent state by using 1000 IU/ml leukemia inhibitory factor (LIF; Chemicon, ESGRO, ESG1107), and grown on top of mouse embryonic fibroblasts feeder layer inactivated by 10 ug/ml of mitomycin C (Sigma, St. L)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 5 micron resolution.
|
| Description |
Gene expression wild type
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.3.1.1 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 026655_D_F_20110722) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Dec 26, 2018 |
| Last update date |
Dec 27, 2018 |
| Contact name |
Seiji Ogawa |
| E-mail(s) |
cfy00450@gmail.com
|
| Organization name |
Keio University
|
| Street address |
Shinanomachi35
|
| City |
Tokyo |
| ZIP/Postal code |
1608582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE124354 |
Mouse ES Cells: Wild type vs. Zscan5b knockout |
|
