J82 cells were maintained MEM medium supplemented with 10% FBS, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 2% MEM vitamin solution, and 100 units/ml penicillin-, and 100 µg/ml streptomycin.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated at 0 hours (no treatment) or and at 0.5, 1, 2, and 3 hours following exposure to frankincense oil using RNeasy® Mini total RNA isolation kit based on manufacture’s procedures.
Label
Cy3
Label protocol
250 ng of RNA from a single cell culture from each time point was labeled using the Illumina Total Prep RNA Amplification Kit following manufacturer’s directions (Ambion, Austin. TX)
Hybridization protocol
cRNA was hybridized overnight on Illumina human Ref-8 version 3 BeadChips. Microarray chips were washed to high stringency and labeled with streptavidin -Cy3 (Amersham Biosciences, Piscataway, NJ) prior to scanning on an Illumina BeadArray Reader.
Scan protocol
Microarrays were scanned on an Illumina BeadArray Reader
Description
None
Data processing
Initial intensity values were obtained using with Illumina Bead Studio software with background subtraction. For normalization, negative values were replaced with 1, and all values were quantile normalized using Matlab software.