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Sample GSM3511174 Query DataSets for GSM3511174
Status Public on Dec 14, 2019
Title rat-drug concetration 3.2g/kg-repH9
Sample type RNA
 
Source name rat-drug concetration 3.2g/kg-kidney
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
sample group: Mongolian medicine Narenmandula group
treatment: Narenmandula 3.2g/kg
tissue: renal tissues
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Ligation Mix Biotin (Affymetrix)
 
Hybridization protocol Following fragmentation, mirRNA targets were hybridized for 16 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Command Console (AGCC)
Description miRNA expression data from drug group
Data processing The data were preprocessed with R/Bioconductor package Affy ,and the processing algorithm used Range Migration Algorithm (RMA)
 
Submission date Dec 13, 2018
Last update date Dec 15, 2019
Contact name Xiulan Wang
E-mail(s) wangxl_1203@163.com
Organization name Inner Mongolia University For The Nationlities
Department Affiliated Hospital
Street address HuoLinhe
City Tongliao
State/province Inner Mongolia
ZIP/Postal code 028000
Country China
 
Platform ID GPL19117
Series (1)
GSE123776 microRNA expression profile in renal tissues of rats with doxorubicin nephropathy by Mongolian medicine Narenmandula

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
14q0_st 0.414984623
14qI-1_st 0.418980807
14qI-1_x_st 0.277246248
14qI-2_st 0.199143248
14qI-3_x_st 0.375650136
14qI-4_st 0.241269364
14qI-4_x_st 0.532141184
14qI-5_st 0.25300278
14qI-6_st 0.299516809
14qI-7_st 0.492700847
14qI-8_st 0.097000365
14qI-8_x_st 0.098343204
14qI-9_x_st 0.083363592
14qII-1_st 0.370784084
14qII-1_x_st 0.603596625
14qII-10_st 0.432392816
14qII-11_st 0.393354199
14qII-12_st 0.426293334
14qII-12_x_st 0.282364274
14qII-13_st 0.108396668

Total number of rows: 36353

Table truncated, full table size 984 Kbytes.




Supplementary file Size Download File type/resource
GSM3511174_H9.CEL.gz 721.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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