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| Status |
Public on Mar 28, 2019 |
| Title |
human astrocyte, rep 1 [HA-1] |
| Sample type |
SRA |
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| Source name |
HA1800, ScienCell
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| Organism |
Homo sapiens |
| Characteristics |
cell line: HA1800
cell type: human fetal cortical astrocytes
passages: p10 - p15
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| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA were extracted according to protocols accompanying with Macherey-Nagel NucleoSpin RNA kit, then sent to sequencing center for polyA enrichment
libraries were constructed using KAPA RNA kit at UCLA Technology Center for Genomics & Bioinformatics
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
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| Data processing |
quality check using FastQC v0.11.3
reads were aligned to hg19 using HISAT2 version 2.0.4
counts summarized using StringTie v1.3.4
Genome_build: hg19
Supplementary_files_format_and_content: for each sample, a tsv file containing FPKM and TPM generated by stringtie
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| Submission date |
Dec 06, 2018 |
| Last update date |
Mar 28, 2019 |
| Contact name |
Gong Chen |
| Organization name |
Penn State University
|
| Street address |
Old Main
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| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platform ID |
GPL21290 |
| Series (1) |
| GSE123397 |
Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways |
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| Relations |
| BioSample |
SAMN10526371 |
| SRA |
SRX5099493 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM3502662_HA-1-abundance.tsv.gz |
677.7 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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