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Sample GSM3502662 Query DataSets for GSM3502662
Status Public on Mar 28, 2019
Title human astrocyte, rep 1 [HA-1]
Sample type SRA
Source name HA1800, ScienCell
Organism Homo sapiens
Characteristics cell line: HA1800
cell type: human fetal cortical astrocytes
passages: p10 - p15
Extracted molecule total RNA
Extraction protocol total RNA were extracted according to protocols accompanying with Macherey-Nagel NucleoSpin RNA kit, then sent to sequencing center for polyA enrichment
libraries were constructed using KAPA RNA kit at UCLA Technology Center for Genomics & Bioinformatics
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Data processing quality check using FastQC v0.11.3
reads were aligned to hg19 using HISAT2 version 2.0.4
counts summarized using StringTie v1.3.4
Genome_build: hg19
Supplementary_files_format_and_content: for each sample, a tsv file containing FPKM and TPM generated by stringtie
Submission date Dec 06, 2018
Last update date Mar 28, 2019
Contact name Gong Chen
Organization name Penn State University
Street address Old Main
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
Platform ID GPL21290
Series (1)
GSE123397 Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways
BioSample SAMN10526371
SRA SRX5099493

Supplementary file Size Download File type/resource
GSM3502662_HA-1-abundance.tsv.gz 677.7 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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