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Sample GSM3494168 Query DataSets for GSM3494168
Status Public on May 01, 2019
Title RNA_GO_P7_Setd2_Con_RNA_rep1
Sample type SRA
Source name oocyte
Organism Mus musculus
Characteristics tissue: oocyte
developmental stage: GO_P7
genotype: Setd2+/-
Treatment protocol Oocytes (20-30 μm) were harvested from postnatal 7 days females for both Setd2 control and mutant in M2 medium.
Extracted molecule total RNA
Extraction protocol The RNA extraction follows smart-seq2 protocol
The RNA-seq libraries were generated following the Smart-seq2 protocol with minor modification (Picelli et al., 2014).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Description processed data file:
Data processing RNA-seq datasets were mapped to the mouse mm9 reference genome by Tophat 2.0.11. Gene expression was then calculated according to refFlat database by cufflinks 2.0.2.
Genome_build: mm9
Supplementary_files_format_and_content: The RNA FPKM txt file contains FPKM value for each sample.
Submission date Nov 27, 2018
Last update date May 01, 2019
Contact name Wei Xie
Organization name Tsinghua University
Department School of Life Science
Street address Haidian District
City Beijing
ZIP/Postal code 100084
Country China
Platform ID GPL21273
Series (2)
GSE112832 SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [RNA-seq]
GSE112835 SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development
BioSample SAMN10490819
SRA SRX5070513

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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