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Sample GSM3487683 Query DataSets for GSM3487683
Status Public on Jul 09, 2019
Title Control rep1
Sample type SRA
 
Source name skin
Organism Homo sapiens
Characteristics cell type: hypertrophic scar fibroblasts
tissue: skin
treatment: control
Treatment protocol Cells were starved for 12h and then treated with pentoxifylline for 24h
Growth protocol Cells were cultured with DMEM/Low Glucose medium
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
Ion Proton Sequencing Protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Ion Torrent Proton
 
Data processing Reads filtering under criteria removing reads with 20% of the base quality lower than 13
Mapsplice v2.2.0 mapping to reference genome
count counting
Genome_build: GRCh38
Supplementary_files_format_and_content: TXT file contain counts for each sample
 
Submission date Nov 25, 2018
Last update date Jul 09, 2019
Contact name fangfang yang
E-mail(s) cy1212@outlook.com
Organization name xijing hospital
Street address No. 127 Changle West Road
City xi’an
ZIP/Postal code 710032
Country China
 
Platform ID GPL17303
Series (1)
GSE122891 Pentoxifylline-induced differentially-expressed genes in hypertrophic scar fibroblasts
Relations
BioSample SAMN10474387
SRA SRX5059154

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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