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Sample GSM3485386 Query DataSets for GSM3485386
Status Public on Jun 28, 2019
Title 99s-3
Sample type SRA
 
Source name H99 strain RNA-seq sample, replicate 3
Organism Cryptococcus neoformans var. grubii H99
Characteristics growth phase: logarithmic phase, OD600=1.0
Extracted molecule total RNA
Extraction protocol For the transcriptome analysis, mice and monkey were sacrificed at 7-day post infection. Lung and brain tissue were isolated and resuspended in RNAlater. Monkey lung tissues were removed from the chest, and cut into 0.5 cm X 0.5 cm X 0.5 cm cubes. Tissues were then processed for RNA-seq analysis. PBS treated mice and monkeys were used as RNA-seq analysis reference samples. Total RNA was isolated with Trizol. RNA library construction and sequencing were generated by using the TruSeq RNA Sample Preparation Kit based on the standard protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to whole genome using bowtie v0.12.2.
Genome_build: Mus_musculus.GRCm38
Genome_build: GCF_000364345.1_Macaca_fascicularis_5.0
Genome_build: Cryptococcus neoformans var. grubii H99
Supplementary_files_format_and_content: *.txt: Tab-delimited text files include NCBI Gene IDs and reads values (raw counts) for each Sample.
 
Submission date Nov 20, 2018
Last update date Jun 30, 2019
Contact name Li Hailong
E-mail(s) lihailong1166@gmail.com
Organization name Northeastern University
Street address NO. 3-11, Wenhua Road, Heping District
City ShenYang
State/province Liaoning
ZIP/Postal code 110001
Country China
 
Platform ID GPL24689
Series (1)
GSE122785 Unveil the transcriptional landscape at the Cryptococcus-host axis in mice and nonhuman primates
Relations
BioSample SAMN10457891
SRA SRX5042751

Supplementary file Size Download File type/resource
GSM3485386_Cn-99s-3.txt.gz 31.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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