NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3478715 Query DataSets for GSM3478715
Status Public on May 20, 2019
Title human astrocyte, rep 3
Sample type SRA
 
Source name HA1800, ScienCell
Organism Homo sapiens
Characteristics cell type: human fetal cortical astrocytes
passages: p10 - p15
Extracted molecule total RNA
Extraction protocol total RNA were extracted according to protocols accompanying with Macherey-Nagel NucleoSpin RNA kit, then sent to sequencing center for polyA enrichment
libraries were constructed using KAPA RNA kit at UCLA Technology Center for Genomics & Bioinformatics
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description HA_3
Data processing quality check using FastQC v0.11.3
reads were aligned to hg19 using HISAT2 version 2.0.4
counts summarized using featureCounts v1.5.1 (count) or StringTie v1.3.4 (RPKM)
differential analysis by DESeq2 v1.16.1
Genome_build: hg19
Supplementary_files_format_and_content: a txt file containing read count from all samples generated by featureCounts
 
Submission date Nov 19, 2018
Last update date May 20, 2019
Contact name Gong Chen
Organization name Penn State University
Street address Old Main
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
 
Platform ID GPL21290
Series (1)
GSE122701 Transcriptome analysis of small molecule-mediated astrocyte-to-neuron reprogramming
Relations
BioSample SAMN10441194
SRA SRX5026193

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap