|Public on Oct 01, 2022
|cell type: epithelial cells
treatment: treatment with RK
|We chose the concentration of 400 μg/mL RK for 72 h to study
|DLD-1 cells were cultured in DMEM medium supplemented with 10% Fetal Bovine Serum, 100 U/mL penicillin and 0.1 mg/mL streptomycin at in a 37 °C humidified atmosphere containing 5% CO2.
|Total RNA was extracted using Total RNA Miniprep Kit (Axygen, Hangzhou, China) according to the supplier’s instruction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|Illumina HiSeq 3000
|Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using TopHat v2.1.0 with default parameters
Transcript assembly and FPKM (RPKM) estimated for RNA-Seq data using Cufflinks v126.96.36.199 with default parameters
Supplementary_files_format_and_content: Excel file includes RPKM values for each Sample ...
|Nov 15, 2018
|Last update date
|Oct 01, 2022
|The Fourth Aﬃliated Hospital of Harbin Medical University
|Department of General Surgery & Bio-Bank of General Surgery
|No. 37 Yiyuan Street
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