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Sample GSM3440008 Query DataSets for GSM3440008
Status Public on Apr 07, 2021
Title G1-5: Colon, AOM+DSS treated, Vehicle control, biological rep.3
Sample type RNA
Source name Colon, AOM treated
Organism Mus musculus
Characteristics strain: C57Bl/6
tissue: Colon
treatment regime: Treated - vehicle control
Treatment protocol Azoxymethane (AOM) was given a single (10mg/kg B.wt) intraperitonielly at day 0 and three bouts of 5% DSS at days 5-10, 25-30, 45-50. At day 140, the mice were sacrificed. Cardamonin was administered orally, at a dose of 10mg/kg, 5 times every week for simultaneous group. And treatment started at a dose of 10mg/kg, 5times every week from 16th week to 30th week for the ''after tumor formation''group.
Growth protocol Colon collected from mice treated with AOM+DSS, followed by Cardamonin simultaneously treated or after tumor development. Colon from normal, healthy mice were used as control.
Extracted molecule total RNA
Extraction protocol Colon dissected and rinsed in 1X PBS, followed by snap freezing in liquid nitrogen. This was ground in liquid nitrogen, and mirVana miRNA isolation kit was used for the subsequent steps.
Label Biotin
Label protocol Total RNA was used for the reaction, Poly(A) tailing was performed followed by biotin labelling of the RNA. This was done by Flash Tag Biotin HSR labelling kit by Affymetrix
Hybridization protocol Samples were hybridised using Affymetrix hybridisation kit. Briefly, biotin labelled sample and hybridisation master mix were incubated at 99oC for 5 mins, then at 45oC for 5 mins. 130ul was injected into the array and both septa covered with tough spots. Loaded into hybridisation oven and incubated at 48oC, 60rpm for 16-18hrs
Scan protocol The chips were then scanned with an Affymetrix GeneChip Scanner 3000 7G (Affymetrix, Santa Clara, California, United States).
Description miRNA expression from AOM and cardamonin treated samples
Data processing The Data generated from the scan was then analyzed using the Bioconductor packages (Oligo, limma)
Submission date Oct 22, 2018
Last update date Apr 07, 2021
Contact name Dr. Harikumar K B
Phone +91-471-2529-596
Organization name Rajiv Gandhi centre for Biotechnology
Department Cancer Research Program
Lab CRP-7
Street address Poojappura
City Thycaud
State/province Kerala
ZIP/Postal code 695 014
Country India
Platform ID GPL19117
Series (1)
GSE121608 Cardamonin repel colitis and its associated colorectal cancer

Data table header descriptions
VALUE RMA signal intensity

Data table
14q0_st 1.279084065
14qI-1_st 1.879709952
14qI-1_x_st 1.821906637
14qI-2_st 0.765812016
14qI-3_x_st 1.637817989
14qI-4_st 0.852690522
14qI-4_x_st 0.751172287
14qI-5_st 1.408028542
14qI-6_st 1.234278009
14qI-7_st 1.696421217
14qI-8_st 1.061599991
14qI-8_x_st 1.303088569
14qI-9_x_st 1.172349118
14qII-1_st 0.923595851
14qII-1_x_st 1.188148503
14qII-10_st 1.210033958
14qII-11_st 0.887793829
14qII-12_st 0.812768901
14qII-12_x_st 0.750852144
14qII-13_st 0.747160198

Total number of rows: 36353

Table truncated, full table size 983 Kbytes.

Supplementary file Size Download File type/resource
GSM3440008_G1-5_miRNA-4_0.CEL.gz 699.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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