NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM343897 Query DataSets for GSM343897
Status Public on Dec 15, 2008
Title 293T cells CBE_2
Sample type RNA
 
Source name 293T cells
Organism Homo sapiens
Characteristics Human Embryonic Kidney 293 cells which also contain the SV40 Large T-antigen.
Treatment protocol Treated with Conduritol B epoxide (CBE) at 100 micromolar for 5 days
Growth protocol Growth in DMEM HG medium, passaged q 2 days.
Extracted molecule total RNA
Extraction protocol Cells homogenized with Qiashredder homogenizer, RNA extracted with RNeasy Mini Kit. RNA verified with NanoDrop ND-1000, Agilent RNA 6000 Nano Assay and agarose electrophoresis.
Label Cy5
Label protocol AminoAllyl aRNA labeling. The reactive amino group of 5-(3-aminoallyl)-UTP/5-(3-aminoallyl)-dUTP was used to conjugate the purified aRNA with the NHS-CyDye.
 
Hybridization protocol Phalanx Hybridization Protocol
Scan protocol Phalanx scanning protocol
Description CBE treated
Data processing Quantile normalization
 
Submission date Nov 19, 2008
Last update date Nov 21, 2008
Contact name Philippe M Campeau
E-mail(s) pcampeau@hotmail.com, philippe.campeau@mail.mcgill.ca
Organization name Baylor College of Medicine
Department Molecular and Human Genetics
Lab Brendan Lee Lab
Street address 1 Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6254
Series (1)
GSE13675 Conduritol B Epoxide treatment of MSCs and 293T cells

Data table header descriptions
ID_REF
VALUE Intensity

Data table
ID_REF VALUE
PH_hs_0018403 26
PH_hs_0026369 26
PH_hs_0028777 26
PH_hs_0004272 28
PH_hs_0026116 46
PH_hs_0034130 46
PH_hs_0002257 48
PH_hs_0004169 48
PH_hs_0027944 48
PH_hs_0027961 48
PH_hs_0028226 48
PH_hs_0028436 48
PH_hs_0028456 48
PH_hs_0040039 48
PH_hs_0000947 67
PH_hs_0015002 67
PH_hs_0023210 67
PH_hs_0024481 67
PH_hs_0025192 67
PH_hs_0028069 67

Total number of rows: 11222

Table truncated, full table size 226 Kbytes.




Supplementary file Size Download File type/resource
GSM343897.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap