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Sample GSM343896 Query DataSets for GSM343896
Status Public on Dec 15, 2008
Title 293T cells CBE_1
Sample type RNA
 
Source name 293T cells
Organism Homo sapiens
Characteristics Human Embryonic Kidney 293 cells which also contains the SV40 Large T-antigen.
Treatment protocol Treated with Conduritol B epoxide (CBE) at 100 micromolar for 5 days
Growth protocol Growth in DMEM HG medium, passaged q 2 days.
Extracted molecule total RNA
Extraction protocol Cells homogenized with Qiashredder homogenizer, RNA extracted with RNeasy Mini Kit. RNA verified with NanoDrop ND-1000, Agilent RNA 6000 Nano Assay and agarose electrophoresis.
Label Cy5
Label protocol AminoAllyl aRNA labeling. The reactive amino group of 5-(3-aminoallyl)-UTP/5-(3-aminoallyl)-dUTP was used to conjugate the purified aRNA with the NHS-CyDye.
 
Hybridization protocol Phalanx Hybridization Protocol
Scan protocol Phalanx scanning protocol
Description CBE treated
Data processing Quantile normalization
 
Submission date Nov 19, 2008
Last update date Nov 21, 2008
Contact name Philippe M Campeau
E-mail(s) pcampeau@hotmail.com, philippe.campeau@mail.mcgill.ca
Organization name Baylor College of Medicine
Department Molecular and Human Genetics
Lab Brendan Lee Lab
Street address 1 Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6254
Series (1)
GSE13675 Conduritol B Epoxide treatment of MSCs and 293T cells

Data table header descriptions
ID_REF
VALUE Intensity

Data table
ID_REF VALUE
PH_hs_0028410 28
PH_hs_0001102 49
PH_hs_0003939 49
PH_hs_0013327 49
PH_hs_0027980 49
PH_hs_0034531 49
PH_hs_0040774 49
PH_hs_0027489 53
PH_hs_0027944 53
PH_hs_0030721 53
PH_hs_0035600 53
PH_hs_0038533 53
PH_hs_0039079 53
PH_hs_0002051 55
PH_hs_0004357 55
PH_hs_0022499 55
PH_hs_0025842 55
PH_hs_0027972 55
PH_hs_0034387 55
PH_hs_0004580 59

Total number of rows: 11222

Table truncated, full table size 226 Kbytes.




Supplementary file Size Download File type/resource
GSM343896.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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