|
| Status |
Public on Apr 25, 2019 |
| Title |
ovarian secondary follicles_9 weeks old mouse, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ovarian secondary follicles (9 weeks old mouse)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ovarian secondary follicles
strain: C57BL/6J
age: 9 weeks old
|
| Growth protocol |
The 9 weeks old and 32 weeks old, specific pathogen-free (SPF), female C57BL/6J mice were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, PR China)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAiso plus reagent extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Command Console Software.
|
| Description |
Gene expression data from ovarian secondary follicles extracted from 9 weeks old mouse
|
| Data processing |
The raw data from microarray analysis was normalized using robust multi-array average (RMA) algorithm.
|
| |
|
| Submission date |
Oct 18, 2018 |
| Last update date |
Apr 25, 2019 |
| Contact name |
Shixuan Wang |
| E-mail(s) |
shixuanwang@tjh.tjmu.edu.cn
|
| Phone |
+86-13995553319
|
| Organization name |
Huazhong University of Science and Technology
|
| Street address |
No.1095 Jie Fang Avenue
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430030 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE121493 |
Expression data from young and old mouse ovarian secondary follicles |
|
