|
| Status |
Public on Nov 07, 2018 |
| Title |
IL-10+ CD4+ T-cells from PBMCs (donor 1) |
| Sample type |
SRA |
| |
|
| Source name |
T-cells from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Libraries were generated using the 10x Genomics Chromium platform
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Pair-ended UMI-indexed Illumina reads were demultiplexed using cellranger mkfastq pipeline,trimmed using Trimmomatic (to 26 bp and 98 bp) followed by standard CellRanger pipeline in order to perform alignment Mm8 respective genome, read filtering and UMI counting for each sample.
Genome_build: hg19
Supplementary_files_format_and_content: Sparse matrix output files from Cellranger
|
| |
|
| Submission date |
Oct 15, 2018 |
| Last update date |
Nov 09, 2018 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
|
| Street address |
Martinistrasse 52
|
| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20301 |
| Series (2) |
| GSE121267 |
Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Human 10x] |
| GSE121393 |
Molecular and functional heterogeneity of IL-10-producing CD4+ T cells |
|
| Relations |
| BioSample |
SAMN10245149 |
| SRA |
SRX4890369 |
