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Sample GSM3408030 Query DataSets for GSM3408030
Status Public on Nov 30, 2018
Title CCR7loCD137hi-1_ RNA
Sample type SRA
Source name Sorted subpopulation (CCR7loCD137hi) of five-day cultured Th1 cells.
Organism Mus musculus
Characteristics cell type: CCR7loCD137hi CD4 T
strain: BALB/c
Treatment protocol None
Growth protocol See "description".
Extracted molecule total RNA
Extraction protocol Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Description Splenic CD4_T cells were stimulated under Th1 culture conditions (15 ng/ml IL-2, 10 ng/ml IL-12) for 4 days in vitro. These cells were separated according to the expression of CCR7 and CD137 by Aria cell sorter.
Data processing Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
Submission date Oct 01, 2018
Last update date Nov 30, 2018
Contact name Yusuke ENDO
Phone 81432262186
Organization name Chiba University
Department Immunology
Lab Immunology
Street address 1-8-1 Inohana, Chuo-ku
City Chiba-city
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
Platform ID GPL18480
Series (2)
GSE120667 Acc1 determines memory potential of individual CD4+ T cells by regulating de novo fatty acid biosynthesis [RNA-seq]
GSE122863 Acc1 determines memory potential of individual CD4+ T cells by regulating de novo fatty acid biosynthesis
BioSample SAMN10147938
SRA SRX4779407

Supplementary file Size Download File type/resource
GSM3408030_CCR7loCD137hi-1_RNA.txt.gz 601.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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