|
| Status |
Public on Jul 01, 2020 |
| Title |
conditioned_2 |
| Sample type |
SRA |
| |
|
| Source name |
T cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: conditioned
naive t cell isolation: CD4+ CD45RO-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells from 24-well plates were harvested by centriguation and RNA was extracted using Qiagen RNeasy Micro Kit.
Standard Illumina protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Conditioned decidual cell medium was added to differentiating Th17 cells
|
| Data processing |
BaseCalls performed using CASAVA
Reads were aligned to the Homo sapiens genome GRCh37.75 using Tophat2 v2.1.1
Aligned reads mapping to each gene were counted using HTSeq-count. GTF file for Ensembl genebuild GRCh37.87 was used.
Supplementary_files_format_and_content: HTseq counts
|
| |
|
| Submission date |
Sep 24, 2018 |
| Last update date |
Jul 02, 2020 |
| Contact name |
Arun Rajendra Chavan |
| E-mail(s) |
arun.chavan@yale.edu
|
| Organization name |
Yale University
|
| Department |
Ecology and Evolutionary Biology
|
| Street address |
850 West Campus Drive
|
| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE120380 |
RNA-seq data of human T cells treated with decidual cell conditioned medium |
|
| Relations |
| BioSample |
SAMN10118457 |
| SRA |
SRX4733666 |
