|
Status |
Public on Sep 21, 2018 |
Title |
Bone marrow W |
Sample type |
SRA |
|
|
Source name |
Bone marrow
|
Organism |
Homo sapiens |
Characteristics |
cell type: bone marrow cells age: 28 years old
|
Treatment protocol |
No treatment
|
Growth protocol |
Using standard operating procedures, mononuclear cells from 20 healthy donors' bone marrow aspirates were isolated using Ficoll density gradient separation and cryopreserved in 90% FBS/ 10% DMSO for storage in 72 liquid nitrogen.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
scRNAseq was performed using 10X Genomics Single Cell 3’ Solution, version 2 according to manufacturer’s instructions (protocol rev A). Droplet-based scRNAseq
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Data processing |
Data were analyzed using Cell Ranger V2.0.0 (10X Genomics). Genome_build: GRCh38 Supplementary_files_format_and_content: Cell Ranger generated one mtx file and two tsv files for gene ID and cell barcode ID.
|
|
|
Submission date |
Sep 19, 2018 |
Last update date |
Sep 24, 2018 |
Contact name |
Laura Dillon |
E-mail(s) |
laura.dillon2@nih.gov
|
Organization name |
National Heart Lung and Blood Institute, NIH
|
Department |
Hematology Branch
|
Lab |
Myeloid Malignancies
|
Street address |
Room 10CRC 6-5142, 10 Center Drive
|
City |
Bethesda |
State/province |
Maryland |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL21290 |
Series (2) |
GSE120221 |
Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry [scRNA] |
GSE120446 |
Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry |
|
Relations |
BioSample |
SAMN10095274 |
SRA |
SRX4720061 |