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Sample GSM3395246 Query DataSets for GSM3395246
Status Public on Sep 20, 2018
Title E9_ST2pos-transfer_Nb_lung_eo_rep2
Sample type SRA
 
Source name eosinophils in lungs
Organism Mus musculus
Characteristics tissue: lung
cell type: eosinophils
strain: BALB/c
Growth protocol Prior to the infection, we transferred ST2+ memory Th2 cells from mice 40 days after infection of 500 organisms of Larvae of Nippostrongylus brasiliensis (Nb). One month later, the mice were infected with 500 organisms of the Larvae. We sorted eosinophils from the lungs of the mice 4 days after the infection.
Extracted molecule total RNA
Extraction protocol Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Data processing Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
 
Submission date Sep 19, 2018
Last update date Sep 24, 2018
Contact name Atsushi Onodera
Organization name Chiba University
Department Immunology
Street address Chuo-ku Inohana 1-8-1
City Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL18480
Series (1)
GSE120173 Characterization of ST2+ and ST2- mTh cells in helminth infection
Relations
BioSample SAMN10091176
SRA SRX4715150

Supplementary file Size Download File type/resource
GSM3395246_E9_ST2pos-transfer_Nb_lung_eo_rep2.txt.gz 174.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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