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Sample GSM3387279 Query DataSets for GSM3387279
Status Public on Sep 14, 2018
Title 10_CD46-deficienct Patient 2
Sample type SRA
Source name CD46-deficienct Patient 2 CD8+ T cells activated with anti-CD3+anti-CD46 6hrs
Organism Homo sapiens
Characteristics file name: EW3541_10
activation state: Anti-CD3+anti-CD46
Extracted molecule total RNA
Extraction protocol CD8+ T cells were isolated from peripheral blood. RNA was extracted using RNeasy Mini kit with on-column DNAse digestion
The sequencing libraries were constructed from 10 pg – 10 ng of total RNA using the Takara Bio USA’s SMART-Seq V4 Ultra Low Input RNA Kit for Sequencing (Cat # 634888) following the manufacturer instruction.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description RNA-seq
Data processing Sequencing reads were trimmed for adaptor contamination with Cutadapt (v1.16)
then aligned to human genome with STAR (v2.5.3a)
read counts were produced by using featureCounts(v.1.5.2)
Genome_build: GENCODE release 26
Submission date Sep 13, 2018
Last update date Sep 15, 2018
Contact name Mehdi Pirooznia
Phone 410-340-6052
Organization name National Institutes of Health
Street address 12 SOUTH DR
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL21290
Series (2)
GSE119918 Transcriptome analysis of CD8+ T cells from healthy controls and patients wth CD46 deficiency
GSE119919 Analysis of transcriptomes of healthy donor and CD46 deficient CD8 T cells
BioSample SAMN10056039
SRA SRX4677052

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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