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Sample GSM3387261 Query DataSets for GSM3387261
Status Public on Jan 07, 2019
Title Lung_ILC2_WT_IL2_7_33_input
Sample type SRA
 
Source name Lung ILC2
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: WT
tissue: Lung
cell type: Group 2 innate lymphoid cells (ILC2s)
antibody: Input
Growth protocol For ChIP-seq, lung ILC2s were cultured with IL-2/IL-7/IL-33 in vitro for 4 weeks, and were cultured with or without IL-33 for a week.
Extracted molecule genomic DNA
Extraction protocol Lysates were purified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
ChIP-seq libraries were created from the DNA with NEBNext ChIP-seq Library Prep Master Mix set for Illumina Kit (NEB).
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 1500
 
Description s13265
Data processing Basecalls performed using CASAVA version 1.8.2.
ChIP sequences were aligned on mm9 using TopHat and bowtie2 (version 2.1.0) with default parameter, respectively.
MACS to call peaks of ChIP-seq and generate wig files of ChIP-seq data.
Genome_build: mm9
Supplementary_files_format_and_content: wig files were generated using MACS.
 
Submission date Sep 13, 2018
Last update date Jan 07, 2019
Contact name Takashi Ebihara
E-mail(s) tebihara@med.akita-u.ac.jp
Phone 81-188846080
Organization name Akita University Graduate School of Medicine
Department Department of Medical Biology
Street address 1-1-1 Hondo
City Akita City
ZIP/Postal code 010-8543
Country Japan
 
Platform ID GPL18480
Series (1)
GSE111871 Runx/Cbfβ complexes protect ILC2s from exhaustion during allergic airway inflammation
Relations
BioSample SAMN10056024
SRA SRX4677015

Supplementary file Size Download File type/resource
GSM3387261_Lung_ILC2_WT_IL2_7_33_input.wig.gz 38.2 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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