|
| Status |
Public on Jan 07, 2019 |
| Title |
Lung_ILC2_WT_IL2_7_33_ChIP-seq_K27ac_rp2 |
| Sample type |
SRA |
| |
|
| Source name |
Lung ILC2
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: WT
tissue: Lung
cell type: Group 2 innate lymphoid cells (ILC2s)
antibody: Rabbit anti-H3K27 acetylation mAb (Cell Signalling #8173)
|
| Growth protocol |
For ChIP-seq, lung ILC2s were cultured with IL-2/IL-7/IL-33 in vitro for 4 weeks, and were cultured with or without IL-33 for a week.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were purified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
ChIP-seq libraries were created from the DNA with NEBNext ChIP-seq Library Prep Master Mix set for Illumina Kit (NEB).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
s13262
|
| Data processing |
Basecalls performed using CASAVA version 1.8.2.
ChIP sequences were aligned on mm9 using TopHat and bowtie2 (version 2.1.0) with default parameter, respectively.
MACS to call peaks of ChIP-seq and generate wig files of ChIP-seq data.
Genome_build: mm9
Supplementary_files_format_and_content: wig files were generated using MACS.
|
| |
|
| Submission date |
Sep 13, 2018 |
| Last update date |
Jan 07, 2019 |
| Contact name |
Takashi Ebihara |
| E-mail(s) |
tebihara@med.akita-u.ac.jp
|
| Phone |
81-188846080
|
| Organization name |
Akita University Graduate School of Medicine
|
| Department |
Department of Medical Biology
|
| Street address |
1-1-1 Hondo
|
| City |
Akita City |
| ZIP/Postal code |
010-8543 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE111871 |
Runx/Cbfβ complexes protect ILC2s from exhaustion during allergic airway inflammation |
|
| Relations |
| BioSample |
SAMN10055983 |
| SRA |
SRX4677012 |
