|Public on Aug 21, 2009
|Lung tissue from Foxa3 KO challenged with OVA
|Total RNA was extracted using Qiagen RNEasy columns. RNA integrity was confirmed using an Agilent 2100 Bioanalyzer
|400ng of RNA was amplified and labeled using the One-Color Agilent Low RNA Input Linear Amplification Kit
|Labeled cRNA was assessed using the Nandrop ND-100 , and equal amounts of Cy3 labeled target were hybridized to Agilent whole genome custom arrays (platform acc# GPL7202). Hybridizations were performed for 14 hrs, according to the manufacturers protocol.
|Arrays were scanned using the Agilent microarray scanner
|11 arrays were hybridized and represent 3 lung samples for groups WT saline, WT OVA and KO OVA. There are 2 samples for the KO saline group.
|Raw signal intensities were extracted with Feature Extraction v9.1 software (Agilent). The dataset was normalized using the quantile normalization method. The median feature pixel intensity was used as the raw signal before normalization and no background subtraction was performed.
|Oct 28, 2008
|Last update date
|Aug 21, 2009
|Andrea J Barczak
|UCSF Sandler Center Functional Genomics Core Facility
|1550 4th St Rock Hall 545
|Distinct roles of FOXA2 and FOXA3 in allergic airway disease