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Sample GSM3375459 Query DataSets for GSM3375459
Status Public on Sep 01, 2021
Title exp_2_adipocytes co-culture
Sample type RNA
Source name WM3682 melanoma line
Organism Homo sapiens
Characteristics cell line: WM3682
protocol: adipocytes co-culture
Treatment protocol Exp.1 - WM1716 melnaoma cells were stably trasfected with scrambled control or miR-211. Exp.2 - WM3682 melnaoma cells were co-cultured with adipocytes or mono-cultured. RNA was extracted at day 5.
Growth protocol Exp.1- WM1716 melanoma cells were grown in 10% serum DMEM. Exp.2- WM3682 melanoma cells were grown in adipocytes medium.
Extracted molecule total RNA
Extraction protocol RNA was isolated from WM1716 and WM3682 cells using Trizol (Qiagen), according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with: miRNA-4_0 or HuGene-1_1-st-v1, as indicated (Affymetrix) and scanned at the HUJI Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RNA expression value derived from Affymetrix Launcher software
Data processing Raw data were processed in R for background correction and RMA normalization. Normalized expression data were log-transformed.
Submission date Sep 05, 2018
Last update date Sep 01, 2021
Contact name Carmit Levy
Organization name Tel Aviv University
Department Human Genetics and Biochemistry
Lab Sackler School of Medicine
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
Platform ID GPL19117
Series (1)
GSE119478 Adipocytes induce melanoma plasticity by sensitizing cells to TGFb

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
14q0_st 0.993512783
14qI-1_st 2.901704554
14qI-1_x_st 1.79005265
14qI-2_st 1.294685129
14qI-3_x_st 1.037674391
14qI-4_st 1.682308825
14qI-4_x_st 1.616647719
14qI-5_st 1.49823726
14qI-6_st 0.871604211
14qI-7_st 1.461287188
14qI-8_st 1.340180753
14qI-8_x_st 1.329467104
14qI-9_x_st 1.242106936
14qII-1_st 0.70467985
14qII-1_x_st 0.691234373
14qII-10_st 1.120746511
14qII-11_st 1.144195547
14qII-12_st 1.423612168
14qII-12_x_st 1.083490667
14qII-13_st 1.213832919

Total number of rows: 36353

Table truncated, full table size 984 Kbytes.

Supplementary file Size Download File type/resource
GSM3375459_4-adipo1_miRNA-4_0_.CEL.gz 714.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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