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Sample GSM3375456 Query DataSets for GSM3375456
Status Public on Sep 01, 2021
Title exp_1_scrambled transfection
Sample type RNA
 
Source name WM1716 melanoma line
Organism Homo sapiens
Characteristics cell line: WM1716
transfection: scrambled control
Treatment protocol Exp.1 - WM1716 melnaoma cells were stably trasfected with scrambled control or miR-211. Exp.2 - WM3682 melnaoma cells were co-cultured with adipocytes or mono-cultured. RNA was extracted at day 5.
Growth protocol Exp.1- WM1716 melanoma cells were grown in 10% serum DMEM. Exp.2- WM3682 melanoma cells were grown in adipocytes medium.
Extracted molecule total RNA
Extraction protocol RNA was isolated from WM1716 and WM3682 cells using Trizol (Qiagen), according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with: miRNA-4_0 or HuGene-1_1-st-v1, as indicated (Affymetrix) and scanned at the HUJI Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RNA expression value derived from Affymetrix Launcher software
Data processing Raw data were processed in R for background correction and RMA normalization. Normalized expression data were log-transformed.
 
Submission date Sep 05, 2018
Last update date Sep 01, 2021
Contact name Carmit Levy
E-mail(s) carmitlevy@post.tau.ac.il
Organization name Tel Aviv University
Department Human Genetics and Biochemistry
Lab Sackler School of Medicine
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platform ID GPL11532
Series (1)
GSE119478 Adipocytes induce melanoma plasticity by sensitizing cells to TGFb

Data table header descriptions
ID_REF
VALUE log2 GC-RMA signal

Data table
ID_REF VALUE
7892501 5.844176815
7892502 3.2689848
7892503 3.066295821
7892504 6.76317876
7892505 2.723200735
7892506 3.712544002
7892507 3.336422187
7892508 4.192845622
7892509 10.7571434
7892510 3.28180046
7892511 3.087824721
7892512 5.119020194
7892513 2.903001929
7892514 10.34922462
7892515 7.817741187
7892516 3.629119133
7892517 4.996161177
7892518 3.272667185
7892519 3.416432109
7892520 7.82770581

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM3375456_M165.ga.cel.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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