|
| Status |
Public on Sep 26, 2018 |
| Title |
Donor1_memory Tregs_activated |
| Sample type |
RNA |
| |
|
| Source name |
memory Tregs_activated
|
| Organism |
Homo sapiens |
| Characteristics |
subject status/id: Healthy Donor 1
sorted cell type: Memory Tregs
treatment: Activated
|
| Treatment protocol |
200,000 naïve, memory and CD161+ Tregs were either sorted from fresh blood directly into Trizol LS (Ambion) for baseline genetic profile or cells were polyclonally activated post-sorting for 4h with Dynabeads Human T-Activator CD3/CD28 (ratio 1:1) and then lyzed in Trizol LS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNeasy mini kit (QIAGEN) and Ovation PicoSL WTA System V2 (NuGEN Technologies) was used for reverse transcription and cDNA amplification steps.
|
| Label |
biotin
|
| Label protocol |
Fragmentation and labelling was performed using the Encore Biotin Module (NuGEN Technologies); all kits were employed following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Samples were run on GeneChip® Human Gene 1.0 ST Array (Affymetrix Ltd).
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Data processing of Affymetrix arrays was carried out using Partek Genome Studio. The data were RMA normalized (including background correction, quantile normalization, Log2 transformation and median polish probeset summarization).
|
| |
|
| Submission date |
Sep 03, 2018 |
| Last update date |
Sep 27, 2018 |
| Contact name |
Mehdi Pirooznia |
| E-mail(s) |
pirooznia@gmail.com
|
| Phone |
410-340-6052
|
| Organization name |
National Institutes of Health
|
| Street address |
12 SOUTH DR
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE119374 |
Transcriptome analysis of naïve Tregs, memory Tregs and CD161+ Tregs |
| GSE119375 |
Analysis of transcriptomes and open chromatin regions of sub-populations of human Tregs |
|
