|
| Status |
Public on Sep 26, 2018 |
| Title |
Healthy donor 2 [BA_2] |
| Sample type |
SRA |
| |
|
| Source name |
healthy donor_CD4+CD25+ T cells
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Healthy Donor
tissue: blood
cell type: CD4+CD25+ T cells
|
| Treatment protocol |
Cells were isolated from freshly drawn blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were captured for each sample and libraries were sequenced on the Illumina Hiseq 3000 instrument.
The experiment was performed using the 10X Genomics’ Chromium Single Cell 3’ gene expression V2 kit following the manufacturer’s instruction. I1: Index, R1: 16 bp cell barcode + 10 bp UMI, R2: Transcript
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
BA-2_S2
|
| Data processing |
10x genomics cell ranger was used for initial analysis
Surat was used for data clustering and expression analysis
|
| |
|
| Submission date |
Sep 03, 2018 |
| Last update date |
Sep 26, 2018 |
| Contact name |
Mehdi Pirooznia |
| E-mail(s) |
pirooznia@gmail.com
|
| Phone |
410-340-6052
|
| Organization name |
National Institutes of Health
|
| Street address |
12 SOUTH DR
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (2) |
| GSE119373 |
single cell RNA-sequencing data from CD4+CD25+ human cells |
| GSE119375 |
Analysis of transcriptomes and open chromatin regions of sub-populations of human Tregs |
|
| Relations |
| BioSample |
SAMN09948035 |
| SRA |
SRX4649754 |
