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Sample GSM3373887 Query DataSets for GSM3373887
Status Public on Sep 26, 2018
Title Healthy donor 1 [BA_1]
Sample type SRA
 
Source name healthy donor_CD4+CD25+ T cells
Organism Homo sapiens
Characteristics subject status: Healthy Donor
tissue: blood
cell type: CD4+CD25+ T cells
Treatment protocol Cells were isolated from freshly drawn blood
Extracted molecule total RNA
Extraction protocol Cells were captured for each sample and libraries were sequenced on the Illumina Hiseq 3000 instrument.
The experiment was performed using the 10X Genomics’ Chromium Single Cell 3’ gene expression V2 kit following the manufacturer’s instruction. I1: Index, R1: 16 bp cell barcode + 10 bp UMI, R2: Transcript
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description BA-1_S1
Data processing 10x genomics cell ranger was used for initial analysis
Surat was used for data clustering and expression analysis
 
Submission date Sep 03, 2018
Last update date Sep 26, 2018
Contact name Mehdi Pirooznia
E-mail(s) pirooznia@gmail.com
Phone 410-340-6052
Organization name National Institutes of Health
Street address 12 SOUTH DR
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL21290
Series (2)
GSE119373 single cell RNA-sequencing data from CD4+CD25+ human cells
GSE119375 Analysis of transcriptomes and open chromatin regions of sub-populations of human Tregs
Relations
BioSample SAMN09948036
SRA SRX4649753

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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