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Sample GSM3369720 Query DataSets for GSM3369720
Status Public on Aug 31, 2021
Title Utx_KO_H3K27me3
Sample type SRA
Source name Utx_KO_Splenic CD8 T cell
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: Utx KO
tissue: spleen
cell type: Naive CD8 T (CD44lowCD62Lhigh) cells
chip antibody: H3K27me3 (abcam, ab6002, lot 205847)
Growth protocol Naïve CD8 T (CD44lowCD62Lhigh) cells were prepared from spleens of WT or Utx KO mice. Naïve CD8 T cells were stimulated with an immobilized anti-TCR-β mAb (3 μg/ml, H57-597; BioLegend) and an anti-CD28 mAb (1 μg/ml, 37.5; BioLegend), and IL-2 (10 ng/ml, Cat.21-8021:Tonbo biosciences) for two days.
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to NEB's instructions accompanying the NEBNext ChIP-Seq Library prep Master Mix Set for Illumina (E6240)
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 1500
Data processing Illumina Casava1.8.2 software used for basecalling.
ChIP-seq reads were aligned to the mm9 genome assembly using bowtie (Galaxy Tool Version 1.1.2)
peaks were called using MACS (Galaxy Tool Version 1.0.1)
All ChIP-seq analysis was performed on Galaxy web site
Genome_build: mm9
Supplementary_files_format_and_content: wig
Submission date Aug 31, 2018
Last update date Aug 31, 2021
Contact name Junpei Suzuki
Organization name Ehime University
Department Graduate School of Medicine
Lab Immunology
Street address Shitukawa
City Toon
ZIP/Postal code 791-0295
Country Japan
Platform ID GPL18480
Series (1)
GSE119312 Histone H3K27 demethylase negatively controls the memory formation of Ag-stimulated CD8+ T cells
BioSample SAMN09940804
SRA SRX4628612

Supplementary file Size Download File type/resource
GSM3369720_Utx_KO.wig.gz 361.2 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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