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Sample GSM3359655 Query DataSets for GSM3359655
Status Public on Aug 31, 2019
Title human patient blood miRNA 6
Sample type RNA
 
Source name human patient blood miRNA
Organism Homo sapiens
Characteristics subject status: Chinese imported falciparum malaria patient
age (years): 27
gender: male
tissue: blood
parasitemia (%): 5.0%
concentration of hemoglobin (g/l): 134
wbc (×10^9/l): 2.86
platelets (×10^9/l): 56
lymphocytes (×10^9/l): 0.78
lymphocyte (%): 27.3
neutrophils (×10^9/l): 1.82
neutrophils (%): 63.4
monocytess (×10^9/l): 0.23
monocyte (%): 7.9
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description miRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
E-mail(s) zouyang.bfh@outlook.com
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
 
Platform ID GPL19117
Series (2)
GSE119151 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [miRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
14q0_st 0.038805388
14qI-1_st 0.41964627
14qI-1_x_st 0.591708418
14qI-2_st 0.122197221
14qI-3_x_st 0.059175102
14qI-4_st 0.00688965
14qI-4_x_st 0.01788704
14qI-5_st 0.477595918
14qI-6_st 0.610559914
14qI-7_st 1.484378256
14qI-8_st 0.431584821
14qI-8_x_st 0.522913179
14qI-9_x_st 0.501910629
14qII-10_st 0.114439355
14qII-11_st 0.221123077
14qII-12_st 0.288772131
14qII-12_x_st 0.648284899
14qII-13_st 0.353562309
14qII-14_st 0.16825114
14qII-14_x_st 0.208040867

Total number of rows: 36353

Table truncated, full table size 985 Kbytes.




Supplementary file Size Download File type/resource
GSM3359655_patient6_miRNA-4_0_.CEL.gz 774.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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