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Sample GSM3359650 Query DataSets for GSM3359650
Status Public on Aug 31, 2019
Title human patient blood miRNA 1
Sample type RNA
 
Source name human patient blood miRNA
Organism Homo sapiens
Characteristics subject status: Chinese imported falciparum malaria patient
age (years): 27
gender: male
tissue: blood
parasitemia (%): 1.0%
concentration of hemoglobin (g/l): 111
wbc (×10^9/l): 3.45
platelets (×10^9/l): 26
lymphocytes (×10^9/l): 0.69
lymphocyte (%): 20
neutrophils (×10^9/l): 2.64
neutrophils (%): 76.5
monocytess (×10^9/l): 0.11
monocyte (%): 3.2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description miRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
E-mail(s) zouyang.bfh@outlook.com
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
 
Platform ID GPL19117
Series (2)
GSE119151 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [miRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
14q0_st 0.338815322
14qI-1_st 0.325695694
14qI-1_x_st 0.257472091
14qI-2_st 0.11230689
14qI-3_x_st 0.257749701
14qI-4_st 0.643775913
14qI-4_x_st 0.757124532
14qI-5_st 0.203073849
14qI-6_st 0.135793369
14qI-7_st 0.795090935
14qI-8_st 0.473015405
14qI-8_x_st 0.49714126
14qI-9_x_st 0.209698289
14qII-10_st 0.636330736
14qII-11_st 0.27016628
14qII-12_st 0.553675288
14qII-12_x_st 0.583076793
14qII-13_st 0.580775274
14qII-14_st 0.605396362
14qII-14_x_st 0.636330736

Total number of rows: 36353

Table truncated, full table size 986 Kbytes.




Supplementary file Size Download File type/resource
GSM3359650_patient1_miRNA-4_0_.CEL.gz 702.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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