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Sample GSM3359647 Query DataSets for GSM3359647
Status Public on Aug 31, 2019
Title human normal blood miRNA 4
Sample type RNA
Source name human normal blood miRNA
Organism Homo sapiens
Characteristics subject status: normal, healthy subject
age (years): 22
gender: male
tissue: blood
concentration of hemoglobin (g/l): 127
wbc (×10^9/l): 7.09
platelets (×10^9/l): 207
lymphocytes (×10^9/l): 2.69
lymphocyte (%): 37.9
neutrophils (×10^9/l): 3.54
neutrophils (%): 49.9
monocytess (×10^9/l): 0.8
monocyte (%): 11.3
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description miRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
Platform ID GPL19117
Series (2)
GSE119151 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [miRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
VALUE RMA signal intensity

Data table
14q0_st 0.206936223
14qI-1_st 0.584999491
14qI-1_x_st 0.546441832
14qI-2_st 0.246517222
14qI-3_x_st 0.172033708
14qI-4_st 0.182301058
14qI-4_x_st 0.110205097
14qI-5_st 0.172175637
14qI-6_st 0.203933696
14qI-7_st 0.71415
14qI-8_st 0.214900355
14qI-8_x_st 0.28358281
14qI-9_x_st 0.369682251
14qII-10_st 0.147184579
14qII-11_st 0.407849673
14qII-12_st 0.481394646
14qII-12_x_st 0.377268303
14qII-13_st 0.364090807
14qII-14_st 0.638035347
14qII-14_x_st 0.177871287

Total number of rows: 36353

Table truncated, full table size 986 Kbytes.

Supplementary file Size Download File type/resource
GSM3359647_normal4_miRNA-4_0_.CEL.gz 723.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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