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Sample GSM3359646 Query DataSets for GSM3359646
Status Public on Aug 31, 2019
Title human normal blood miRNA 3
Sample type RNA
 
Source name human normal blood miRNA
Organism Homo sapiens
Characteristics subject status: normal, healthy subject
age (years): 44
gender: male
tissue: blood
concentration of hemoglobin (g/l): 156
wbc (×10^9/l): 6.48
platelets (×10^9/l): 200
lymphocytes (×10^9/l): 1.81
lymphocyte (%): 27.9
neutrophils (×10^9/l): 4.2
neutrophils (%): 64.8
monocytess (×10^9/l): 0.39
monocyte (%): 6
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description miRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
E-mail(s) zouyang.bfh@outlook.com
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
 
Platform ID GPL19117
Series (2)
GSE119151 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [miRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
14q0_st 0.650724192
14qI-1_st 0.365369177
14qI-1_x_st 0.326811517
14qI-2_st 0.008816501
14qI-3_x_st 0.365337491
14qI-4_st 0.20044291
14qI-4_x_st 0.313791528
14qI-5_st 0.575145856
14qI-6_st 0.192997733
14qI-7_st 0.443485265
14qI-8_st 0.311574974
14qI-8_x_st 0.815610169
14qI-9_x_st 0.1062079
14qII-10_st 0.104606739
14qII-11_st 0.426910743
14qII-12_st 0.634186824
14qII-12_x_st 0.668114221
14qII-13_st 0.368380415
14qII-14_st 0.791449596
14qII-14_x_st 0.046770369

Total number of rows: 36353

Table truncated, full table size 986 Kbytes.




Supplementary file Size Download File type/resource
GSM3359646_normal3_miRNA-4_0_.CEL.gz 701.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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